Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes. Evidence for O-linked GlcNAc.
CR Torres, GW Hart - Journal of Biological Chemistry, 1984 - ASBMB
Bovine milk galactosyltransferase has been used, in conjunction with UDP-[3H] galactose,
as an impermeant probe for accessible GlcNAc residues on the surfaces of lymphocytes.
Galactosylation of living thymic lymphocytes is dependent upon cell number, enzyme
concentration, UDP-galactose concentration, and Mn2+ concentration. Kinetics of labeling
are biphasic, leveling off at approximately 30 min. The data strongly indicate vectorial
surface labeling and covalent attachment of galactose. Thymocytes, T-lymphocytes, and B …
as an impermeant probe for accessible GlcNAc residues on the surfaces of lymphocytes.
Galactosylation of living thymic lymphocytes is dependent upon cell number, enzyme
concentration, UDP-galactose concentration, and Mn2+ concentration. Kinetics of labeling
are biphasic, leveling off at approximately 30 min. The data strongly indicate vectorial
surface labeling and covalent attachment of galactose. Thymocytes, T-lymphocytes, and B …