Mitochondrial fission is a crucial cellular process mediated by the mechanoenzymatic
GTPase, dynamin-related protein 1 (Drp1). During mitochondrial division, Drp1 is recruited
from the cytosol to the outer mitochondrial membrane by one, or several, integral membrane
proteins. One such Drp1 partner protein, mitochondrial fission factor (Mff), is essential for
mitochondrial division, but its mechanism of action remains unexplored. Previous studies
have been limited by a weak interaction between Drp1 and Mff in vitro. Through refined in …