Skewed TCRVβ repertoire in human thymus persists after thymic emigration: influence of genomic imposition, thymic maturation and environmental challenge on …
C Reinhardt, A Melms - Immunobiology, 1998 - Elsevier
In order to investigate the mechanisms involved in originating a diverse TCR repertoire in
human peripheral blood we analyzed TCRVβ surface expression in different T cell subsets
of unrelated individuals. The relative frequencies of 11 distinct V (3 chains were determined
for immature double positive (DP) as well as for mature CD4 single positive (4SP) and CD8
single positive (8SP) thymocytes, respectively. By comparing these data with expression in
peripheral blood T lymphocytes of the same donors we were able to show that usage of …
human peripheral blood we analyzed TCRVβ surface expression in different T cell subsets
of unrelated individuals. The relative frequencies of 11 distinct V (3 chains were determined
for immature double positive (DP) as well as for mature CD4 single positive (4SP) and CD8
single positive (8SP) thymocytes, respectively. By comparing these data with expression in
peripheral blood T lymphocytes of the same donors we were able to show that usage of …