Oropharyngeal aspiration: an alternative route for challenging in a mouse model of chemical-induced asthma
V De Vooght, JAJ Vanoirbeek, S Haenen, E Verbeken… - Toxicology, 2009 - Elsevier
BACKGROUND: To assess the importance of the route of challenge in an existing mouse
model of chemical-induced asthma, we replaced intranasal instillation by oropharyngeal
aspiration. To our knowledge, oropharyngeal aspiration as a challenge route has not yet
been investigated in a mouse model of chemical-induced asthma. METHODS: On days 1
and 8, mice were dermally sensitized with toluene diisocyanate (TDI)(0.3%)[or vehicle
(acetone/olive oil)] and on day 15 they received a single challenge, via oropharyngeal …
model of chemical-induced asthma, we replaced intranasal instillation by oropharyngeal
aspiration. To our knowledge, oropharyngeal aspiration as a challenge route has not yet
been investigated in a mouse model of chemical-induced asthma. METHODS: On days 1
and 8, mice were dermally sensitized with toluene diisocyanate (TDI)(0.3%)[or vehicle
(acetone/olive oil)] and on day 15 they received a single challenge, via oropharyngeal …