NF-κB controls expression of inhibitor IκBα: evidence for an inducible autoregulatory pathway

SC Sun, PA Ganchi, DW Ballard, WC Greene - Science, 1993 - science.org
SC Sun, PA Ganchi, DW Ballard, WC Greene
Science, 1993science.org
The eukaryotic transcription factor nuclear factor-kappa B (NF-κB) participates in many parts
of the genetic program mediating T lymphocyte activation and growth. Nuclear expression of
NF-κB occurs after its induced dissociation from its cytoplasmic inhibitor IκBα. Phorbol ester
and tumor necrosis factor-alpha induction of nuclear NF-κB is associated with both the
degradation of performed IκBα and the activation of IκBα gene expression. Transfection
studies indicate that the IκBα gene is specifically induced by the 65-kilodalton transactivating …
The eukaryotic transcription factor nuclear factor-kappa B (NF-κB) participates in many parts of the genetic program mediating T lymphocyte activation and growth. Nuclear expression of NF-κB occurs after its induced dissociation from its cytoplasmic inhibitor IκBα. Phorbol ester and tumor necrosis factor-alpha induction of nuclear NF-κB is associated with both the degradation of performed IκBα and the activation of IκBα gene expression. Transfection studies indicate that the IκBα gene is specifically induced by the 65-kilodalton transactivating subunit of NF-κB. Association of the newly synthesized IκBα with p65 restores intracellular inhibition of NF-κB DNA binding activity and prolongs the survival of this labile inhibitor. Together, these results show that NF-κB controls the expression of IκBα by means of an inducible autoregulatory pathway.
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