Systemic innate immune activation in food protein–induced enterocolitis syndrome
R Goswami, AB Blazquez, R Kosoy, A Rahman… - Journal of Allergy and …, 2017 - Elsevier
Background Food protein–induced enterocolitis syndrome (FPIES) is a non–IgE-mediated
food allergy of infancy whose pathophysiology is poorly understood. Objectives We set out
to identify and phenotype allergen-responsive cells in peripheral blood of a cohort of
subjects undergoing supervised food challenge for FPIES. Methods We profiled antigen-
responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained
before and after challenge by CyTOF mass cytometry and RNAseq. Results Using a CD154 …
food allergy of infancy whose pathophysiology is poorly understood. Objectives We set out
to identify and phenotype allergen-responsive cells in peripheral blood of a cohort of
subjects undergoing supervised food challenge for FPIES. Methods We profiled antigen-
responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained
before and after challenge by CyTOF mass cytometry and RNAseq. Results Using a CD154 …