Freshly isolated tumor-specific endothelial cells (TEC) can be used to explore molecular
mechanisms of tumor angiogenesis and serve as an in vitro model for developing new
angiogenesis inhibitors for cancer. However, long-term in vitro expansion of murine
endothelial cells (EC) is challenging due to phenotypic drift in culture (endothelial-to-
mesenchymal transition) and contamination with non-EC. This is especially true for TEC
which are readily outcompeted by co-purified fibroblasts or tumor cells in culture. Here, a …