Immunohistochemical staining of B7-H1 (PD-L1) on paraffin-embedded slides of pancreatic adenocarcinoma tissue

E Bigelow, KM Bever, H Xu, A Yager, A Wu… - JoVE (Journal of …, 2013 - jove.com
B7-H1/PD-L1, a member of the B7 family of immune-regulatory cell-surface proteins, plays
an important role in the negative regulation of cell-mediated immune responses through its
interaction with its receptor, programmed death-1 (PD-1) 1, 2. Overexpression of B7-H1 by
tumor cells has been noted in a number of human cancers, including melanoma,
glioblastoma, and carcinomas of the lung, breast, colon, ovary, and renal cells, and has
been shown to impair anti-tumor T-cell immunity3-8. Recently, B7-H1 expression by …