Isolation, purification and labeling of mouse bone marrow neutrophils for functional studies and adoptive transfer experiments
M Swamydas, MS Lionakis - JoVE (Journal of Visualized Experiments), 2013 - jove.com
Neutrophils are critical effector cells of the innate immune system. They are rapidly recruited
at sites of acute inflammation and exert protective or pathogenic effects depending on the
inflammatory milieu. Nonetheless, despite the indispensable role of neutrophils in immunity,
detailed understanding of the molecular factors that mediate neutrophils' effector and
immunopathogenic effects in different infectious diseases and inflammatory conditions is still
lacking, partly because of their short half life, the difficulties with handling of these cells and …
at sites of acute inflammation and exert protective or pathogenic effects depending on the
inflammatory milieu. Nonetheless, despite the indispensable role of neutrophils in immunity,
detailed understanding of the molecular factors that mediate neutrophils' effector and
immunopathogenic effects in different infectious diseases and inflammatory conditions is still
lacking, partly because of their short half life, the difficulties with handling of these cells and …