An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues
P Cohen, S Klumpp, DL Schelling - FEBS letters, 1989 - Wiley Online Library
P Cohen, S Klumpp, DL Schelling
FEBS letters, 1989•Wiley Online LibraryThe type 2A protein phosphatases in mammalian tissue extracts are inhibited completely
and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly
affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These
observations have been exploited to develop an improved procedure for the identification
and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.
and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly
affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These
observations have been exploited to develop an improved procedure for the identification
and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.
The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1–2 nMadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μMadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.
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