Transcriptome profiling of human FoxP3+ regulatory T cells
R Bhairavabhotla, YC Kim, DD Glass, TM Escobar… - Human immunology, 2016 - Elsevier
The major goal of this study was to perform an in depth characterization of the “gene
signature” of human FoxP3+ T regulatory cells (Tregs). Highly purified Tregs and T
conventional cells (Tconvs) from multiple healthy donors (HD), either freshly explanted or
activated in vitro, were analyzed via RNA sequencing (RNA-seq) and gene expression
changes validated using the nCounter system. Additionally, we analyzed microRNA
(miRNA) expression using TaqMan low-density arrays. Our results confirm previous studies …
signature” of human FoxP3+ T regulatory cells (Tregs). Highly purified Tregs and T
conventional cells (Tconvs) from multiple healthy donors (HD), either freshly explanted or
activated in vitro, were analyzed via RNA sequencing (RNA-seq) and gene expression
changes validated using the nCounter system. Additionally, we analyzed microRNA
(miRNA) expression using TaqMan low-density arrays. Our results confirm previous studies …