An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues

MR Corces, AE Trevino, EG Hamilton, PG Greenside… - Nature …, 2017 - nature.com
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling
that works across multiple applications with substantial improvement of signal-to-
background ratio and information content. The Omni-ATAC protocol generates chromatin
accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the
activities of disease-associated DNA elements in distinct human brain structures. The Omni-
ATAC protocol enables the interrogation of personal regulomes in tissue context and …
Abstract
We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
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