Integrins regulate adhesion-dependent growth, survival and invasion of tumor cells. In particular, expression of integrin α_vβ₃ is associated with progression of a variety of human tumors. Here we reveal a previously undescribed adhesion-independent role for integrin α_vβ₃ in pancreatic cancer and other carcinomas. Specifically, α_vβ₃ expressed in carcinoma cells enhanced anchorage-independent tumor growth in vitro and increased lymph node metastases in vivo. These effects required recruitment of c-Src to the β₃ integrin cytoplasmic tail, leading to c-Src activation, Crk-associated substrate (CAS) phosphorylation and tumor cell survival that, unexpectedly, was independent of cell adhesion or focal adhesion kinase (FAK) activation. Pharmacological blockade of c-Src kinase activity or decreased expression of endogenous α_vβ₃ integrin or c-Src not only inhibited anchorage-independent growth but also suppressed metastasis in vivo, yet these manipulations did not affect tumor cell migration or invasion. These data define an unexpected role for an integrin as a mediator of anchorage independence, suggesting that an α_vβ₃–c-Src signaling module may account for the aggressive behavior of integrin α_vβ₃–expressing tumors in humans.