Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol

RC MacPhail, J Brooks, DL Hunter, B Padnos, TD Irons… - Neurotoxicology, 2009 - Elsevier
The increasing use of zebrafish (Danio rerio) in developmental research highlights the need
for a detailed understanding of their behavior. We studied the locomotion of individual
zebrafish larva (6 days post-fertilization) in 96-well microtiter plates. Movement was
recorded using a video-tracking system. Time of day results indicated locomotion, tested in
darkness (infrared), decreased gradually from early morning to a stable level between 13:
00 and 15: 30h. All further studies were conducted in early-to-late afternoon and lasted …