Introduction of the emm6 gene into an emm-deleted strain of Streptococcus pyogenes restores its ability to resist phagocytosis
J Perez-Casal, MG Caparon, JR Scott - Research in microbiology, 1992 - Elsevier
To complete the formal proof that the M protein functions to protect the group A
streptococcus from phagocytosis and to lay the groundwork for the molecular genetic
dissection of this major virulence factor, we have reintroduced a wild-type allele of the M
protein gene (emm) into the chromosome of a strain from which it had previously been
deleted. For this purpose, we used homologous recombination with an engineered plasmid
that was introduced into the Streptococcus pyogenes chromosome by an electroporation …
streptococcus from phagocytosis and to lay the groundwork for the molecular genetic
dissection of this major virulence factor, we have reintroduced a wild-type allele of the M
protein gene (emm) into the chromosome of a strain from which it had previously been
deleted. For this purpose, we used homologous recombination with an engineered plasmid
that was introduced into the Streptococcus pyogenes chromosome by an electroporation …