Methods for the production of helper-dependent adenoviral vectors
JML Doux, DJ Palmer, P Ng - Gene Therapy Protocols: Production and In …, 2008 - Springer
JML Doux, DJ Palmer, P Ng
Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer …, 2008•SpringerHelper-dependent adenoviral vectors (HDAd) are deleted of all viral coding sequences and
have demonstrated tremendous potential for gene therapy by providing long-term, high-level
transgene expression in the absence of chronic toxicity. Thus, HDAd are superior to early
generation Ad for gene therapy of genetic diseases where long-term transgene expression
is required. This chapter describes in detail the rescue, amplification, and large-scale
production of HDAd.
have demonstrated tremendous potential for gene therapy by providing long-term, high-level
transgene expression in the absence of chronic toxicity. Thus, HDAd are superior to early
generation Ad for gene therapy of genetic diseases where long-term transgene expression
is required. This chapter describes in detail the rescue, amplification, and large-scale
production of HDAd.
Summary
Helper-dependent adenoviral vectors (HDAd) are deleted of all viral coding sequences and have demonstrated tremendous potential for gene therapy by providing long-term, high-level transgene expression in the absence of chronic toxicity. Thus, HDAd are superior to early generation Ad for gene therapy of genetic diseases where long-term transgene expression is required. This chapter describes in detail the rescue, amplification, and large-scale production of HDAd.
Springer