[HTML][HTML] Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer

A Lindsley, P Snider, H Zhou, R Rogers, J Wang… - Developmental …, 2007 - Elsevier
A Lindsley, P Snider, H Zhou, R Rogers, J Wang, M Olaopa, A Kruzynska-Frejtag…
Developmental biology, 2007Elsevier
Periostin is a fasciclin-containing adhesive glycoprotein that facilitates the migration and
differentiation of cells that have undergone epithelial–mesenchymal transformation during
embryogenesis and in pathological conditions. Despite the importance of post-
transformational differentiation as a general developmental mechanism, little is known how
periostin's embryonic expression is regulated. To help resolve this deficiency, a 3.9-kb
periostin proximal promoter was isolated and shown to drive tissue-specific expression in …
Periostin is a fasciclin-containing adhesive glycoprotein that facilitates the migration and differentiation of cells that have undergone epithelial–mesenchymal transformation during embryogenesis and in pathological conditions. Despite the importance of post-transformational differentiation as a general developmental mechanism, little is known how periostin's embryonic expression is regulated. To help resolve this deficiency, a 3.9-kb periostin proximal promoter was isolated and shown to drive tissue-specific expression in the neural crest-derived Schwann cell lineage and in a subpopulation of periostin-expressing cells in the cardiac outflow tract endocardial cushions. In order to identify the enhancer and associated DNA binding factor(s) responsible, in vitro promoter dissection was undertaken in a Schwannoma line. Ultimately a 304-bpperi enhancer was identified and shown to be capable of recapitulating 3.9 kbperi-lacZin vivo spatiotemporal patterns. Further mutational and EMSA analysis helped identify a minimal 37-bp region that is bound by the YY1 transcription factor. The 37-bp enhancer was subsequently shown to be essential for in vivo 3.9 kbperi-lacZ promoter activity. Taken together, these studies identify an evolutionary-conserved YY1-binding 37-bp region within a 304-bp periostin core enhancer that is capable of regulating simultaneous novel tissue-specific periostin expression in the cardiac outflow-tract cushion mesenchyme and Schwann cell lineages.
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