Reduction of tubular flow rate as a mechanism of oliguria in the early phase of endotoxemia revealed by intravital imaging

D Nakano, K Doi, H Kitamura, T Kuwabara… - Journal of the …, 2015 - journals.lww.com
Urine output is widely used as a criterion for the diagnosis of AKI. Although several potential
mechanisms of septic AKI have been identified, regulation of urine flow after glomerular
filtration has not been evaluated. This study evaluated changes in urine flow in mice with
septic AKI. The intratubular urine flow rate was monitored in real time by intravital imaging
using two-photon laser microscopy. The tubular flow rate, as measured by freely filtered dye
(FITC-inulin or Lucifer yellow), time-dependently declined after LPS injection. At 2 hours, the …