[PDF][PDF] AID-dependent activation of a MYC transgene induces multiple myeloma in a conditional mouse model of post-germinal center malignancies

M Chesi, DF Robbiani, M Sebag, WJ Chng, M Affer… - Cancer cell, 2008 - cell.com
M Chesi, DF Robbiani, M Sebag, WJ Chng, M Affer, R Tiedemann, R Valdez, SE Palmer…
Cancer cell, 2008cell.com
By misdirecting the activity of Activation-Induced Deaminase (AID) to a conditional MYC
transgene, we have achieved sporadic, AID-dependent MYC activation in germinal center B
cells of Vk∗ MYC mice. Whereas control C57BL/6 mice develop benign monoclonal
gammopathy with age, all Vk∗ MYC mice progress to an indolent multiple myeloma
associated with the biological and clinical features highly characteristic of the human
disease. Furthermore, antigen-dependent myeloma could be induced by immunization with …
Summary
By misdirecting the activity of Activation-Induced Deaminase (AID) to a conditional MYC transgene, we have achieved sporadic, AID-dependent MYC activation in germinal center B cells of VkMYC mice. Whereas control C57BL/6 mice develop benign monoclonal gammopathy with age, all VkMYC mice progress to an indolent multiple myeloma associated with the biological and clinical features highly characteristic of the human disease. Furthermore, antigen-dependent myeloma could be induced by immunization with a T-dependent antigen. Consistent with these findings in mice, more frequent MYC rearrangements, elevated levels of MYC mRNA, and MYC target genes distinguish human patients with multiple myeloma from individuals with monoclonal gammopathy, implicating a causal role for MYC in the progression of monoclonal gammopathy to multiple myeloma.
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