Neurobehavioral and electroencephalographic abnormalities in Ube3aMaternal-deficient mice

K Miura, T Kishino, E Li, H Webber, P Dikkes… - Neurobiology of …, 2002 - Elsevier
K Miura, T Kishino, E Li, H Webber, P Dikkes, GL Holmes, J Wagstaff
Neurobiology of disease, 2002Elsevier
Angelman syndrome (AS), characterized by motor dysfunction, mental retardation, and
seizures, is caused by several genetic etiologies involving chromosome 15q11–q13,
including mutations of the UBE3A gene. UBE3A encodes UBE3A/E6-AP, a ubiquitin-protein
ligase, and shows brain-specific imprinting, with brain expression predominantly from the
maternal allele. Lack of a functional maternal allele of UBE3A causes AS. In order to
understand the causal relationship between maternal UBE3A mutations and AS, we have …
Angelman syndrome (AS), characterized by motor dysfunction, mental retardation, and seizures, is caused by several genetic etiologies involving chromosome 15q11–q13, including mutations of the UBE3A gene. UBE3A encodes UBE3A/E6-AP, a ubiquitin-protein ligase, and shows brain-specific imprinting, with brain expression predominantly from the maternal allele. Lack of a functional maternal allele of UBE3A causes AS. In order to understand the causal relationship between maternal UBE3A mutations and AS, we have constructed a mouse model with targeted inactivation of Ube3a. The inactive allele contains a lacZ reporter gene for analysis of brain-specific imprinting. Maternal, but not paternal, transmission of the targeted allele leads to β-galactosidase activity in hippocampal and cerebellar neurons. Maternal inheritance of the Ube3a mutant allele also causes impaired performance in tests of motor function and spatial learning, as well as abnormal hippocampal EEG recordings. As predicted from the dependence of UBE3A-mediated ubiquitination of p53 on HPV E6 protein, our maternal-deficient mice show normal brain p53 levels.
Elsevier