[HTML][HTML] Different regions in activation function-1 of the human estrogen receptor required for antiestrogen-and estradiol-dependent transcription activation

EM McInerney, BS Katzenellenbogen - Journal of Biological Chemistry, 1996 - ASBMB
The human estrogen receptor (ER) is a ligand-inducible transcription factor that contains two
transcriptional activation functions, one located in the NH 2-terminal region of the protein (AF-
1) and the second in the COOH-terminal region (AF-2). Antiestrogens, such as trans-
hydroxytamoxifen (TOT), have partial agonistic activity in certain cell types, and studies have
implied that this agonism is AF-1-dependent. We have made progressive NH 2-terminal and
other segment deletions and ligations in the A/B domain, and studied the transcriptional …