Selection of CD8+ PD-1+ lymphocytes in fresh human melanomas enriches for tumor-reactive T cells

T Inozume, K Hanada, QJ Wang… - Journal of …, 2010 - journals.lww.com
Abstract CD8+ tumor-infiltrating lymphocytes (TILs) in human melanomas express high
levels of PD-1 and are functionally impaired. However, adoptive cell therapy using in vitro-
expanded TIL can be a highly effective therapy for patients with advanced melanoma. This
discrepancy led us to further analyze the CD8+ PD-1+ TILs. We found that the percentage of
PD-1− expressing CD8+ T cells was higher in the tumor digests that generate tumor-reactive
TILs after in vitro culture in interleukin-2 (P= 0.0007). Also sorted and expanded CD8+ PD …