cAMP-and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human …
E Butt, K Abel, M Krieger, D Palm, V Hoppe… - Journal of Biological …, 1994 - ASBMB
The vasodilator-stimulated phosphoprotein (VASP) is a major substrate for cAMP-dependent-
(cAK) and cGMP-dependent protein kinase (cGK) in human platelets and other
cardiovascular cells. To identify the VASP phosphorylation sites, purified VASP was
phosphorylated by either protein kinase and subjected to trypsin, V8 and Lys-C proteolysis.
The phosphorylated proteolytic fragments obtained were separated by reversed phase high
performance liquid chromatography. Sequence analysis of the phosphorylated peptides and …
(cAK) and cGMP-dependent protein kinase (cGK) in human platelets and other
cardiovascular cells. To identify the VASP phosphorylation sites, purified VASP was
phosphorylated by either protein kinase and subjected to trypsin, V8 and Lys-C proteolysis.
The phosphorylated proteolytic fragments obtained were separated by reversed phase high
performance liquid chromatography. Sequence analysis of the phosphorylated peptides and …