Using quantitative real-time PCR to determine donor cell engraftment in a competitive murine bone marrow transplantation model
N An, Y Kang - JoVE (Journal of Visualized Experiments), 2013 - jove.com
Murine bone marrow transplantation models provide an important tool in measuring
hematopoietic stem cell (HSC) functions and determining genes/molecules that regulate
HSCs. In these transplant model systems, the function of HSCs is determined by the ability of
these cells to engraft and reconstitute lethally irradiated recipient mice. Commonly, the
donor cell contribution/engraftment is measured by antibodies to donor-specific cell surface
proteins using flow cytometry. However, this method heavily depends on the specificity and …
hematopoietic stem cell (HSC) functions and determining genes/molecules that regulate
HSCs. In these transplant model systems, the function of HSCs is determined by the ability of
these cells to engraft and reconstitute lethally irradiated recipient mice. Commonly, the
donor cell contribution/engraftment is measured by antibodies to donor-specific cell surface
proteins using flow cytometry. However, this method heavily depends on the specificity and …