Cloning and nucleotide sequence of the structural gene encoding for human tryptophanyl-tRNA synthetase

LY Frolova, MA Sudomoina, AY Grigorieva… - Gene, 1991 - Elsevier
LY Frolova, MA Sudomoina, AY Grigorieva, OL Zinovieva, LL Kisselev
Gene, 1991Elsevier
A structural gene encoding bovine (b) tryptophanyl-tRNA synthetase (WRS) has recently
been cloned and sequenced [Garret et al., Biochemistry 30 (1991) 7809–7817]. Using part
of this sequence as a hybridisation probe we have cloned and sequenced a structural gene
encoding human polypeptide highly homologous with two mammalian proteins, bWRS
[Garret et al., Biochemistry 30 (1991) 7809–7817; EMBL accession No. X52113] and rabbit
peptide chain release factor [Lee et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3508–3512] …
Abstract
A structural gene encoding bovine (b) tryptophanyl-tRNA synthetase (WRS) has recently been cloned and sequenced [Garret et al., Biochemistry 30 (1991) 7809–7817]. Using part of this sequence as a hybridisation probe we have cloned and sequenced a structural gene encoding human polypeptide highly homologous with two mammalian proteins, bWRS [Garret et al., Biochemistry 30 (1991) 7809–7817; EMBL accession No. X52113] and rabbit peptide chain release factor [Lee et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3508–3512]. Identification of the sequence encoding a human WRS is based on (i) the presence of ‘HIGH’ and ‘KMSKS’ structural motifs typical for class-I aminoacyl-tRNA synthetases [Eriani et al., Nature 347 (1990) 203–206]; (ii) coincidence of the number of SH groups per subunit estimated experimentally [Muench et al., Science 187 (1975) 1089–1091] and deduced from the cDNA seqeuence (six in both cases); (iii) close resemblance of two WRS polypeptides sequenced earlier [Muench et al., Science 187 (1975) 1089–1091] and the predicted structure in two different regions.
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