Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice
A Kuma, N Mizushima - Autophagy, 2008 - Taylor & Francis
A Kuma, N Mizushima
Autophagy, 2008•Taylor & FrancisGFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal
mapping of the GFP-LC3 transgene in this mouse model. The transgene was located in a
very distal region of chromosome 2, immediately upstream of a putative open reading frame
that is thought to be a part of a pseudogene. With this information, we were able to generate
a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3tg/tg)
were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in …
mapping of the GFP-LC3 transgene in this mouse model. The transgene was located in a
very distal region of chromosome 2, immediately upstream of a putative open reading frame
that is thought to be a part of a pseudogene. With this information, we were able to generate
a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3tg/tg)
were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in …
GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP-LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3tg/tg) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.
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