[HTML][HTML] α-Hemoglobin-stabilizing protein (AHSP) perturbs the proximal heme pocket of oxy-α-hemoglobin and weakens the iron-oxygen bond
CF Dickson, AM Rich, WMH D'Avigdor… - Journal of Biological …, 2013 - ASBMB
α-Hemoglobin (αHb)-stabilizing protein (AHSP) is a molecular chaperone that assists
hemoglobin assembly. AHSP induces changes in αHb heme coordination, but how these
changes are facilitated by interactions at the αHb· AHSP interface is not well understood. To
address this question we have used NMR, x-ray absorption spectroscopy, and ligand
binding measurements to probe αHb conformational changes induced by AHSP binding.
NMR chemical shift analyses of free CO-αHb and CO-αHb· AHSP indicated that the seven …
hemoglobin assembly. AHSP induces changes in αHb heme coordination, but how these
changes are facilitated by interactions at the αHb· AHSP interface is not well understood. To
address this question we have used NMR, x-ray absorption spectroscopy, and ligand
binding measurements to probe αHb conformational changes induced by AHSP binding.
NMR chemical shift analyses of free CO-αHb and CO-αHb· AHSP indicated that the seven …