Multiplication of human immunodeficiency virus in primary cultures of human Kupffer cells—possible role of liver macrophage infection in the physiopathology of AIDS
MP Schmitt, AM Steffan, JL Gendrault, D Jaeck… - Research in …, 1990 - Elsevier
In primary cultures of Kupffer cells obtained from surgical biopsies of human liver by
collagenase perfusion followed by centrifugal elutriation and infected with HIV, the virus
multiplied abundantly, as attested by the appearance of a reverse transcriptase activity in the
medium. Examined by electron microscopy, the cells were found to contain viral particles
with typical features of Lentivirinae. Furthermore, the virus could be revealed by
immunofluorescence using an HIV+ patient serum. HIV antibodies also neutralized the …
collagenase perfusion followed by centrifugal elutriation and infected with HIV, the virus
multiplied abundantly, as attested by the appearance of a reverse transcriptase activity in the
medium. Examined by electron microscopy, the cells were found to contain viral particles
with typical features of Lentivirinae. Furthermore, the virus could be revealed by
immunofluorescence using an HIV+ patient serum. HIV antibodies also neutralized the …