[HTML][HTML] DLX1 acts as a crucial target of FOXM1 to promote ovarian cancer aggressiveness by enhancing TGF-β/SMAD4 signaling

DW Chan, WWY Hui, JJ Wang, MMH Yung, LMN Hui… - Oncogene, 2017 - nature.com
DW Chan, WWY Hui, JJ Wang, MMH Yung, LMN Hui, Y Qin, RR Liang, THY Leung, D Xu
Oncogene, 2017nature.com
Recent evidence from a comprehensive genome analysis and functional studies have
revealed that FOXM1 is a crucial metastatic regulator that drives cancer progression.
However, the regulatory mechanism by which FOXM1 exerts its metastatic functions in
cancer cells remains obscure. Here, we report that DLX1 acts as a FOXM1 downstream
target, exerting pro-metastatic function in ovarian cancers. Both FOXM1 isoforms (FOXM1B
or FOXM1C) could transcriptionally upregulate DLX1 through two conserved binding sites …
Abstract
Recent evidence from a comprehensive genome analysis and functional studies have revealed that FOXM1 is a crucial metastatic regulator that drives cancer progression. However, the regulatory mechanism by which FOXM1 exerts its metastatic functions in cancer cells remains obscure. Here, we report that DLX1 acts as a FOXM1 downstream target, exerting pro-metastatic function in ovarian cancers. Both FOXM1 isoforms (FOXM1B or FOXM1C) could transcriptionally upregulate DLX1 through two conserved binding sites, located at+ 61 to+ 69bp downstream (TFBS1) and− 675 to− 667bp upstream (TFBS2) of the DLX1 promoter, respectively. This regulation was further accentuated by the significant correlation between the nuclear expression of FOXM1 and DLX1 in high-grade serous ovarian cancers. Functionally, the ectopic expression of DLX1 promoted ovarian cancer cell growth, cell migration/invasion and intraperitoneal dissemination of ovarian cancer in mice, whereas small interfering RNA-mediated DLX1 knockdown in FOXM1-overexpressing ovarian cancer cells abrogated these oncogenic capacities. In contrast, depletion of FOXM1 by shRNAi only partially attenuated tumor growth and exerted almost no effect on cell migration/invasion and the intraperitoneal dissemination of DLX1-overexpressing ovarian cancer cells. Furthermore, the mechanistic studies showed that DLX1 positively modulates transforming growth factor-β (TGF-β) signaling by upregulating PAI-1 and JUNB through direct interaction with SMAD4 in the nucleus upon TGF-β1 induction. Taken together, these data strongly suggest that DLX1 has a pivotal role in FOXM1 signaling to promote cancer aggressiveness through intensifying TGF-β/SMAD4 signaling in high-grade serous ovarian cancer cells.
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