[PDF][PDF] Research Article Automatic Generation of Validated Specific Epitope Sets

SC Pro, J Sidney, S Paul, CL Arlehamn, D Weiskopf… - 2015 - academia.edu
2015academia.edu
Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity,
allergies, immunity to pathogens, and host-pathogen interactions and assist in the design
and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to
elucidate a method to select validated reference sets of epitopes to allow detection of T and
B cells. However, the evergrowing information contained in the Immune Epitope Database
(IEDB) and the differences in quality and subjects studied between epitope assays make this …
Accurate measurement of B and T cell responses is a valuable tool to study autoimmunity, allergies, immunity to pathogens, and host-pathogen interactions and assist in the design and evaluation of T cell vaccines and immunotherapies. In this context, it is desirable to elucidate a method to select validated reference sets of epitopes to allow detection of T and B cells. However, the evergrowing information contained in the Immune Epitope Database (IEDB) and the differences in quality and subjects studied between epitope assays make this task complicated. In this study, we develop a novel method to automatically select reference epitope sets according to a categorization system employed by the IEDB. From the sets generated, three epitope sets (EBV, mycobacteria and dengue) were experimentally validated by detection of T cell reactivity ex vivo from human donors. Furthermore, a web application that will potentially be implemented in the IEDB was created to allow users the capacity to generate customized epitope sets.
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