[PDF][PDF] Selective utilization of Toll-like receptor and MyD88 signaling in B cells for enhancement of the antiviral germinal center response

B Hou, P Saudan, G Ott, ML Wheeler, M Ji, L Kuzmich… - Immunity, 2011 - cell.com
B Hou, P Saudan, G Ott, ML Wheeler, M Ji, L Kuzmich, LM Lee, RL Coffman, MF Bachmann
Immunity, 2011cell.com
The contribution of Toll-like receptor (TLR) signaling to T cell-dependent (TD) antibody
responses was assessed by using mice lacking the TLR signaling adaptor MyD88 in
individual cell types. When a soluble TLR9 ligand was used as adjuvant for a protein
antigen, MyD88 was required in dendritic cells but not in B cells to enhance the TD antibody
response, regardless of the inherent immunogenicity of the antigen. In contrast, a TLR9
ligand contained within a virus-like particle substantially augmented the TD germinal center …
Summary
The contribution of Toll-like receptor (TLR) signaling to T cell-dependent (TD) antibody responses was assessed by using mice lacking the TLR signaling adaptor MyD88 in individual cell types. When a soluble TLR9 ligand was used as adjuvant for a protein antigen, MyD88 was required in dendritic cells but not in B cells to enhance the TD antibody response, regardless of the inherent immunogenicity of the antigen. In contrast, a TLR9 ligand contained within a virus-like particle substantially augmented the TD germinal center IgG antibody response, and this augmentation required B cell MyD88. The ability of B cells to discriminate between antigens based on the physical form of a TLR ligand probably reflects an adaptation to facilitate strong antiviral antibody responses.
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