Yeast cells from a wild type or protease-deficient strain were lysed in the absence or
presence of protease inhibitors and the extracts analyzed by analytical high pressure liquid
chromatography on diethylaminoethyl silica gel. Conditions that inhibited protease action
caused elution of a novel DNA polymerase activity at a position in the gradient distinct from
the elution positions of both DNA polymerase I and II. In large scale purifications, this DNA
polymerase, called DNA polymerase III, copurified with a single-stranded DNA dependent 3' …