[HTML][HTML] Unique error signature of the four-subunit yeast DNA polymerase ϵ
PV Shcherbakova, YI Pavlov, O Chilkova… - Journal of Biological …, 2003 - ASBMB
We have purified wild type and exonuclease-deficient four-subunit DNA polymerase ϵ (Pol
ϵ) complex from Saccharomyces cerevisiae and analyzed the fidelity of DNA synthesis by
the two enzymes. Wild type Pol ϵ synthesizes DNA accurately, generating single-base
substitutions and deletions at average error rates of≤ 2× 10-5 and≤ 5× 10-7, respectively.
Pol ϵ lacking 3′→ 5′ exonuclease activity is less accurate to a degree suggesting that
wild type Pol ϵ proofreads at least 92% of base substitution errors and at least 99% of …
ϵ) complex from Saccharomyces cerevisiae and analyzed the fidelity of DNA synthesis by
the two enzymes. Wild type Pol ϵ synthesizes DNA accurately, generating single-base
substitutions and deletions at average error rates of≤ 2× 10-5 and≤ 5× 10-7, respectively.
Pol ϵ lacking 3′→ 5′ exonuclease activity is less accurate to a degree suggesting that
wild type Pol ϵ proofreads at least 92% of base substitution errors and at least 99% of …