A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs
Nucleic acids research, 1996•academic.oup.com
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which
express either Cre-or FLP-recombinase. Plasmids containing authentic recognition targets
for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate
strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of
constructs that are designed for use in Cre-or FLP-mediated genomic manipulations.
express either Cre-or FLP-recombinase. Plasmids containing authentic recognition targets
for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate
strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of
constructs that are designed for use in Cre-or FLP-mediated genomic manipulations.
Abstract
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase (loxPs or FRTs) are recombined when propagated in the appropriate strain. 294-Cre and 294-FLP thus provide a simple test for the recombination competence of constructs that are designed for use in Cre- or FLP-mediated genomic manipulations.
Oxford University Press