[HTML][HTML] The quaternary structure of DNA polymerase ε from Saccharomyces cerevisiae
O Chilkova, BH Jonsson, E Johansson - Journal of Biological Chemistry, 2003 - ASBMB
DNA polymerase ε (Pol ε) fromSaccharomyces cerevisiae consists of four subunits (Pol2,
Dpb2, Dpb3, and Dpb4) and is essential for chromosomal DNA replication. Biochemical
characterizations of Pol ε have been cumbersome due to protease sensitivity and the limited
amounts of Pol ε in cells. We have developed a protocol for overexpression and purification
of Pol ε from S. cerevisiae. The native four-subunit complex was purified to homogeneity by
conventional chromatography. Pol ε was characterized biochemically by sedimentation …
Dpb2, Dpb3, and Dpb4) and is essential for chromosomal DNA replication. Biochemical
characterizations of Pol ε have been cumbersome due to protease sensitivity and the limited
amounts of Pol ε in cells. We have developed a protocol for overexpression and purification
of Pol ε from S. cerevisiae. The native four-subunit complex was purified to homogeneity by
conventional chromatography. Pol ε was characterized biochemically by sedimentation …