Rhodamine-labeled phalloidin staining of morphologically differentiated 3T3L1 adipocytes
demonstrated that F-actin predominantly exists juxtaposed to and lining the inner face of the
plasma membrane (cortical actin) with a smaller amount of stress fiber and/or ruffling actin
confined to the cell bottom in contact with the substratum. The extent of cortical actin
disruption with various doses of either latrunculin B or Clostridium difficiletoxin B (a Rho
family small GTP-binding protein toxin) directly correlated with the inhibition of insulin …