A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity

SS Lee, RYN Lee, AG Fraser, RS Kamath, J Ahringer… - Nature …, 2003 - nature.com
Nature genetics, 2003nature.com
We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans
genes for gene inactivations that increase lifespan. We found that genes important for
mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan.
A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase
gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan.
The long-lived worms with impaired mitochondria had lower ATP content and oxygen …
Abstract
We report a systematic RNA interference (RNAi) screen of 5,690 Caenorhabditis elegans genes for gene inactivations that increase lifespan. We found that genes important for mitochondrial function stand out as a principal group of genes affecting C. elegans lifespan. A classical genetic screen identified a mutation in the mitochondrial leucyl-tRNA synthetase gene (lrs-2) that impaired mitochondrial function and was associated with longer-lifespan. The long-lived worms with impaired mitochondria had lower ATP content and oxygen consumption, but differential responses to free-radical and other stresses. These data suggest that the longer lifespan of C. elegans with compromised mitochrondria cannot simply be assigned to lower free radical production and suggest a more complex coupling of metabolism and longevity.
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