Chronic graft-versus-host disease (cGVHD) is an autoimmune-like syndrome mediated by pathogenic CD4⁺ T and B cells, but the function of extrafollicular and germinal center CD4⁺ T and B interactions in cGVHD pathogenesis remains largely unknown. Here we show that extrafollicular CD4⁺ T and B interactions are sufficient for inducing cGVHD, while germinal center formation is dispensable. The pathogenesis of cGVHD is associated with the expansion of extrafollicular CD44^hiCD62^loPSGL-1^loCD4⁺ (PSGL-1^loCD4⁺) T cells. These cells express high levels of ICOS, and the blockade of ICOS/ICOSL interaction prevents their expansion and ameliorates cGVHD. Expansion of PSGL-1^loCD4⁺ T cells is also prevented by BCL6 or Stat3 deficiency in donor CD4⁺ T cells, with the induction of cGVHD ameliorated by BCL6 deficiency and completely suppressed by Stat3 deficiency in donor CD4⁺ T cells. These results support that Stat3- and BCL6-dependent extrafollicular CD4⁺ T and B interactions play critical functions in the pathogenesis of cGVHD.