[HTML][HTML] Transcriptional regulation of Bim by FoxO3A mediates hepatocyte lipoapoptosis
FJ Barreyro, S Kobayashi, SF Bronk… - Journal of Biological …, 2007 - ASBMB
Hepatocyte lipoapoptosis, a critical feature of nonalcoholic steatohepatitis, can be replicated
in vitro by incubating hepatocytes with saturated free fatty acids (FFA). These toxic FFA
induce Bim expression, which is requisite for their cytotoxicity. Because the FoxO3a
transcription factor has been implicated in Bim expression, our aim was to determine if FFA
induce Bim by a FoxO3a-dependent mechanism. In Huh-7 cells, the saturated FFA, palmitic
and stearic acid, increased Bim mRNA 16-fold. Treatment of cells with the saturated FFA …
in vitro by incubating hepatocytes with saturated free fatty acids (FFA). These toxic FFA
induce Bim expression, which is requisite for their cytotoxicity. Because the FoxO3a
transcription factor has been implicated in Bim expression, our aim was to determine if FFA
induce Bim by a FoxO3a-dependent mechanism. In Huh-7 cells, the saturated FFA, palmitic
and stearic acid, increased Bim mRNA 16-fold. Treatment of cells with the saturated FFA …