[HTML][HTML] NAD kinase levels control the NADPH concentration in human cells

N Pollak, M Niere, M Ziegler - Journal of Biological Chemistry, 2007 - ASBMB
NAD kinases (NADKs) are vital, as they generate the cellular NADP pool. As opposed to
three compartment-specific isoforms in plants and yeast, only a single NADK has been
identified in mammals whose cytoplasmic localization we established by
immunocytochemistry. To understand the physiological roles of the human enzyme, we
generated and analyzed cell lines stably deficient in or overexpressing NADK. Short hairpin
RNA-mediated down-regulation led to similar (about 70%) decrease of both NADK …