[HTML][HTML] SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers

S Zanivan, F Maione, MY Hein… - Molecular & Cellular …, 2013 - ASBMB
Proteomics has been successfully used for cell culture on dishes, but more complex cellular
systems have proven to be challenging and so far poorly approached with proteomics.
Because of the complexity of the angiogenic program, we still do not have a complete
understanding of the molecular mechanisms involved in this process, and there have been
no in depth quantitative proteomic studies. Plating endothelial cells on matrigel recapitulates
aspects of vessel growth, and here we investigate this mechanism by using a spike-in SILAC …