Omega-3 fatty acid supplementation influences the whole blood transcriptome in women with obesity, associated with pro-resolving lipid mediator production
A Polus, B Zapala, U Razny, A Gielicz… - … et Biophysica Acta (BBA …, 2016 - Elsevier
A Polus, B Zapala, U Razny, A Gielicz, B Kiec-Wilk, M Malczewska-Malec, M Sanak…
Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2016•ElsevierThe n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA) may reduce low-grade inflammation associated with obesity.
The relationship between therapeutic response to n-3 PUFAs and modification of the
transcriptome in obesity or metabolic syndrome remains to be explored. Blood samples
were obtained from women with obesity before and after three-months supplementation with
a moderate dose of n-3 PUFAs (1.8 g EPA+ DHA per day) or from controls. n-3 PUFAs (GC) …
docosahexaenoic acid (DHA) may reduce low-grade inflammation associated with obesity.
The relationship between therapeutic response to n-3 PUFAs and modification of the
transcriptome in obesity or metabolic syndrome remains to be explored. Blood samples
were obtained from women with obesity before and after three-months supplementation with
a moderate dose of n-3 PUFAs (1.8 g EPA+ DHA per day) or from controls. n-3 PUFAs (GC) …
Abstract
The n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) may reduce low-grade inflammation associated with obesity. The relationship between therapeutic response to n-3 PUFAs and modification of the transcriptome in obesity or metabolic syndrome remains to be explored.
Blood samples were obtained from women with obesity before and after three-months supplementation with a moderate dose of n-3 PUFAs (1.8 g EPA + DHA per day) or from controls. n-3 PUFAs (GC) and plasma concentrations of lipoxins, resolvins, protectin X (GC–MS/MS) and inflammatory markers (ELISA) were measured. Whole blood transcriptome was assayed using microarray.
Women supplemented with n-3 PUFAs for 3 months had significantly higher levels of EPA and DHA in plasma phosphatidylcholine. n-3 PUFA supplementation, in contrast to placebo, significantly decreased the concentrations of several inflammatory markers (SELE, MCP-1, sVCAM-1, sPECAM-1, and hsCRP), fasting triglycerides and insulin and increased the concentrations of pro-resolving DHA derivatives in plasma. The microarray data demonstrated effects of n-3 PUFAs on PPAR-α, NRF2 and NF-κB target genes.
N-3 PUFAs increased DHA-derived pro-resolving mediators in women with obesity. Elevated resolvins and up-regulation of the resolvin receptor occurred in parallel with activation of PPAR-α target genes related to lipid metabolism and of NRF2 up-regulated antioxidant enzymes.
Elsevier