Studies on the mechanism of antitumor effect of bleomycin on squamous cell carcinoma
H Umezawa, T TAKEUCHI, S HORI, T SAWA… - The Journal of …, 1972 - jstage.jst.go.jp
H Umezawa, T TAKEUCHI, S HORI, T SAWA, M ISHIZUKA, T ICHIKAWA, T KOMAI
The Journal of Antibiotics, 1972•jstage.jst.go.jpMaterials and Methods 3H-Bleomycins: Bleomycin A2 and bleomycin mixture which were
prepared by Nipponkayaku Co. weretritiated and purified by CM-Sephadex chromatography
as reported by Umezawaet aPJ The 3H-bleomycin mixture consisted of 48.5% A2, 27.0%
B2, ll. 8% Al9 3.4% Bi, 3.9% A2'and 3.4% B4. Tritiated bleomycins decompose during
storage and the decomposition can be shownby thin-layer chromatography using a solvent
system of 10% ammoniumacetate-10% ammonia-methanol (9: 1: 10). Therefore, the …
prepared by Nipponkayaku Co. weretritiated and purified by CM-Sephadex chromatography
as reported by Umezawaet aPJ The 3H-bleomycin mixture consisted of 48.5% A2, 27.0%
B2, ll. 8% Al9 3.4% Bi, 3.9% A2'and 3.4% B4. Tritiated bleomycins decompose during
storage and the decomposition can be shownby thin-layer chromatography using a solvent
system of 10% ammoniumacetate-10% ammonia-methanol (9: 1: 10). Therefore, the …
Materials and Methods 3H-Bleomycins: Bleomycin A2 and bleomycin mixture which were prepared by Nipponkayaku Co. weretritiated and purified by CM-Sephadex chromatography as reported by Umezawaet aPJ The 3H-bleomycin mixture consisted of 48.5% A2, 27.0% B2, ll. 8% Al9 3.4% Bi, 3.9% A2'and 3.4% B4. Tritiated bleomycins decompose during storage and the decomposition can be shownby thin-layer chromatography using a solvent system of 10% ammoniumacetate-10% ammonia-methanol (9: 1: 10). Therefore, the bleomycins were employedwithin twoweeksafter their purification.
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