Involvement of prenylated proteins in calcium signaling induced by LTD4 in differentiated U937 cells
V Capra, MR Accomazzo, S Ravasi, M Parenti… - Prostaglandins & other …, 2003 - Elsevier
We investigated signal transduction pathways for LTD4 in the human promonocytic cell line
U937 known, upon differentiation, to express CysLT1 receptors. We confirmed the presence
of high-affinity binding sites for 3H-LTD4, which, in functional studies, displayed the features
of CysLT1 receptor. In fact, three potent and selective CysLT1 receptor antagonists were
able to completely inhibit LTD4-induced response. In turn, cytosolic Ca2+ ([Ca2+] i) increase
(EC50= 3.4 nM±27% CV) was only partially sensitive to pertussis toxin (PTx) as well as to …
U937 known, upon differentiation, to express CysLT1 receptors. We confirmed the presence
of high-affinity binding sites for 3H-LTD4, which, in functional studies, displayed the features
of CysLT1 receptor. In fact, three potent and selective CysLT1 receptor antagonists were
able to completely inhibit LTD4-induced response. In turn, cytosolic Ca2+ ([Ca2+] i) increase
(EC50= 3.4 nM±27% CV) was only partially sensitive to pertussis toxin (PTx) as well as to …