[HTML][HTML] Munc18c interaction with syntaxin 4 monomers and SNARE complex intermediates in GLUT4 vesicle trafficking

M D'Andrea-Merrins, L Chang, AD Lam… - Journal of biological …, 2007 - ASBMB
In the process of insulin-stimulated GLUT4 vesicle exocytosis, Munc18c has been proposed
to control SNARE complex formation by inactivating syntaxin 4 in a self-associated
conformation. Using in vivo fluorescence resonance energy transfer in 3T3L1 adipocytes, co-
immunoprecipitation, and in vitro binding assays, we provide data to indicate that Munc18c
also associates with nearly equal affinity to a mutant of syntaxin 4 in a constitutively open
(unfolded) state (L173A/E174A; LE). To bind to the open conformation of syntaxin 4, we …