Eicosanoid-dependent and-independent formation of individual [14C] stearoyl-labelled lysophospholipids in collagen-stimulated human platelets
LM Thomas, BJ Holub - Biochimica et Biophysica Acta (BBA)-Lipids and …, 1991 - Elsevier
Low level collagen activation of platelets is mediated via the release of arachidonic acid
(AA) from membrane phosphoiipids and the formation of thromboxane A 2 (TxA 2). To
assess the specific phospholipids undergoing deacylation via phospholipase A 2 thereby
providing source (s) of releasable AA, we have measured the individual lysophospholipid
formations in platelets prelabelled with [14 C] stearic acid and incubated with a low level (2
μg/ml) or a high level (10 μg/ml) of collagen in the absence or presence of BW755C, a dual …
(AA) from membrane phosphoiipids and the formation of thromboxane A 2 (TxA 2). To
assess the specific phospholipids undergoing deacylation via phospholipase A 2 thereby
providing source (s) of releasable AA, we have measured the individual lysophospholipid
formations in platelets prelabelled with [14 C] stearic acid and incubated with a low level (2
μg/ml) or a high level (10 μg/ml) of collagen in the absence or presence of BW755C, a dual …