[HTML][HTML] Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)

O Werz, D Szellas, D Steinhilber, O Rådmark - Journal of biological …, 2002 - ASBMB
We demonstrated previously that 5-lipoxygenase (5-LO), a key enzyme in leukotriene
biosynthesis, can be phosphorylated by p38 MAPK-regulated MAPKAP kinases (MKs). Here
we show that mutation of Ser-271 to Ala in 5-LO abolished MK2 catalyzed phosphorylation
and clearly reduced phosphorylation by kinases prepared from stimulated
polymorphonuclear leukocytes and Mono Mac 6 cells. Compared with heat shock protein 27
(Hsp-27), 5-LO was a weak substrate for MK2. However, the addition of unsaturated fatty …