Characterization of how DNA modifications affect DNA binding by C2H2 zinc finger proteins
A Patel, H Hashimoto, X Zhang, X Cheng - Methods in enzymology, 2016 - Elsevier
Much is known about vertebrate DNA methylation and oxidation; however, much less is
known about how modified cytosine residues within particular sequences are recognized.
Among the known methylated DNA-binding domains, the Cys2-His2 zinc finger (ZnF) protein
superfamily is the largest with hundreds of members, each containing tandem ZnFs ranging
from 3 to> 30 fingers. We have begun to biochemically and structurally characterize these
ZnFs not only on their sequence specificity but also on their sensitivity to various DNA …
known about how modified cytosine residues within particular sequences are recognized.
Among the known methylated DNA-binding domains, the Cys2-His2 zinc finger (ZnF) protein
superfamily is the largest with hundreds of members, each containing tandem ZnFs ranging
from 3 to> 30 fingers. We have begun to biochemically and structurally characterize these
ZnFs not only on their sequence specificity but also on their sensitivity to various DNA …