[PDF][PDF] Regulation of microRNA expression and abundance during lymphopoiesis

S Kuchen, W Resch, A Yamane, N Kuo, Z Li… - Immunity, 2010 - cell.com
S Kuchen, W Resch, A Yamane, N Kuo, Z Li, T Chakraborty, L Wei, A Laurence, T Yasuda
Immunity, 2010cell.com
Although the cellular concentration of miRNAs is critical to their function, how miRNA
expression and abundance are regulated during ontogeny is unclear. We applied miRNA-,
mRNA-, and ChIP-Seq to characterize the microRNome during lymphopoiesis within the
context of the transcriptome and epigenome. We show that lymphocyte-specific miRNAs are
either tightly controlled by polycomb group-mediated H3K27me3 or maintained in a semi-
activated epigenetic state prior to full expression. Because of miRNA biogenesis, the cellular …
Summary
Although the cellular concentration of miRNAs is critical to their function, how miRNA expression and abundance are regulated during ontogeny is unclear. We applied miRNA-, mRNA-, and ChIP-Seq to characterize the microRNome during lymphopoiesis within the context of the transcriptome and epigenome. We show that lymphocyte-specific miRNAs are either tightly controlled by polycomb group-mediated H3K27me3 or maintained in a semi-activated epigenetic state prior to full expression. Because of miRNA biogenesis, the cellular concentration of mature miRNAs does not typically reflect transcriptional changes. However, we uncover a subset of miRNAs for which abundance is dictated by miRNA gene expression. We confirm that concentration of 5p and 3p miRNA strands depends largely on free energy properties of miRNA duplexes. Unexpectedly, we also find that miRNA strand accumulation can be developmentally regulated. Our data provide a comprehensive map of immunity's microRNome and reveal the underlying epigenetic and transcriptional forces that shape miRNA homeostasis.
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