CD155/PVR enhances glioma cell dispersal by regulating adhesion signaling and focal adhesion dynamics

KE Sloan, JK Stewart, AF Treloar, RT Matthews… - Cancer research, 2005 - AACR
KE Sloan, JK Stewart, AF Treloar, RT Matthews, DG Jay
Cancer research, 2005AACR
We recently identified the immunoglobulin-CAM CD155/PVR (the poliovirus receptor) as a
regulator of cancer invasiveness and glioma migration, but the mechanism through which
CD155/PVR controls these processes is unknown. Here, we show that expression of
CD155/PVR in rat glioma cells that normally lack this protein enhances their dispersal both
in vitro and on primary brain tissue. CD155/PVR expression also reduced substrate
adhesion, cell spreading, focal adhesion density, and the number of actin stress fibers in a …
Abstract
We recently identified the immunoglobulin-CAM CD155/PVR (the poliovirus receptor) as a regulator of cancer invasiveness and glioma migration, but the mechanism through which CD155/PVR controls these processes is unknown. Here, we show that expression of CD155/PVR in rat glioma cells that normally lack this protein enhances their dispersal both in vitro and on primary brain tissue. CD155/PVR expression also reduced substrate adhesion, cell spreading, focal adhesion density, and the number of actin stress fibers in a substrate-dependent manner. Furthermore, we found that expression of CD155/PVR increased Src/focal adhesion kinase signaling in a substrate-dependent manner, enhancing the adhesion-induced activation of paxillin and p130Cas in cells adhering to vitronectin. Conversely, depletion of endogenous CD155/PVR from human glioma cells inhibited their migration, increased cell spreading, and down-regulated the same signaling pathway. These findings implicate CD155/PVR as a regulator of adhesion signaling and suggest a pathway through which glioma and other cancer cells may acquire a dispersive phenotype.
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